时晰理学博士高聘研究员硕士生导师
1.教育背景
200309-200706齐齐哈尔大学生物工程学士
200709-201006齐齐哈尔大学遗传学硕士
201009-201306东北师范大学细胞生物学博士
2.科研情况
目前累计主持国自然青年基金1项,国家博士后基金1项,海南省面上项目1项,江苏省高校重点1项,江苏省高校面上1项,徐州市课题2项。累计以第一或通讯作者发表SCI论文20余篇,累计影响因子>100,参与编著十四五高等教育药学专业《细胞生物学》教材1部(第11章);获批发明专利4项,实用新型专利1项;累计指导研究所博士研究生2人,硕士研究生近20人。徐州医科大学耳鼻喉科学专业“骨干教师”。
3.研究领域
主要从事交叉组学策略在耳鼻咽喉疾病诊断,靶点筛选,疗效预判及个体化用药的相关研究,曾作为外聘专家,负责“国家耳鼻咽喉临床医学研究中心”自动化精准医疗医学大数据平台建设。部分研究成果已发表于“molecule cancer"(IF=41.444);此外,同时致力于听觉保护及内耳靶向给药分子机制研究,在听觉系统跨血迷路屏障无创药物递送领域,原创性证明LRP1受体是可控状态下开启BLB的关键“钥匙”,该研究已发表于高水平学术期刊Signal Transduction and Targeted Therapy(IF=38.104),并获批发明专利一项。
4.社会兼职
江苏省六大人才高峰
Journal of Otology 编委
中华耳科学杂志编委
徐州医科大学校报青年编委
徐州医科大学(临床学院) 客座硕士生导师
5.代表性项目
1)国家自然科学基金委员会,青年基金项目,81800916,IETP2短肽偶联Klotho蛋白跨越血迷路屏障延缓老年性聋及分子机制研究,201901~202112,21万;
2)中国博士后科学基金委员会,中国博士后科学基金第57批面上资助,201601~201812,5万;
3)江苏省教育部,江苏省高等学校自然科学研究重大项目,19KJA560002,干细胞辅助CI移植增效改善瓦氏综合症猪听功能机制研究,201907~202206,30万;
4)江苏省教育部,江苏省高校自然科学研究面项目,16KJB320016,Cas9介导听神经病小鼠SLC17A8突变基因治疗,201607~201906,6万;
5)江苏省科技厅,江苏省六大人才高峰,WSN-043,IRAK-4在新生小鼠CMV感染性耳聋中的作用,201407~201806,6万;
6)徐州市科技厅,徐州市推动科技创新专项资金青年人才,KC20177,RLA8平衡炎症与自噬间Cross-talk效应改善噪声性听力损伤机制研究,202009~202208,10万;
7)徐州市科技厅,徐州市推动科技创新专项资金项目,KC17087,腺病毒CRISPR/Cas9系统介导OTOF突变听神经病小鼠内耳基因治疗研究,201707~201906,10万.
8)海南省科技厅,海南省自然科学基金(面上项目),NeuroD1介导小鼠SGN原位胶质-神经细胞重编程改善CMV诱发感音神经性聋机制研究,2024.01~2026.12,8万
6.代表性成果
1)Shi Xi†;Wang Zihao†; Ren Wei†......Zhong Wu*; Lin Jian*; and Yang Shiming*; LDL receptor-related protein 1 (LRP1), a novel target for opening the blood-labyrinth barrier (BLB), Signal Transduction and Targeted Therapy, 2022, 7:175. (SCI, IF=38.104, 中科院大类一区)
2)Lu Cheng†;Shi Xi†; Wang Amanda Y†,......Hu Hongyi*; Liu Liangfa*. RNA-Seq profiling of circular RNAs in human laryngeal squamous cell carcinomas[J]. Molecular cancer, 2018, 17(1): 86. (SCI, IF=41.444, 中科院大类一区)
3)Li Rifei†; Zhu Xinjie†......Shi Xi*. Generation of a high-affinity nanobody against CD147 for tumor targeting and therapeutic efficacy through conjugating doxorubicin. Frontiers in Immunology, 2022, 13:852700. (SCI, IF=8.786, 中科院大类二区 )
4)Li Yalan†; Li Shengsheng†, Wu Liyuan†......Shi X*, Qiao Y*. Sestrin 2 deficiency exacerbates noise-induced cochlear injury through inhibiting ULK1/Parkin-mediated mitophagy. Antioxidants & Redox Signaling. 2023, 38 (1-3):115-136. (SCI, IF=7.468, 中科院大类二区 )
5)Chen M†, Li M†, Wu L†......Lin C*,Shi X*, Yang S*. Oridonin employs interleukin 1 receptor type 2 to treat noise-induced hearing loss by blocking inner ear inflammation. Biochemical Pharmacology. 2023, 210: 115457. DOI:10.1016/j.bcp.2023.115457 .(SCI, IF=6.1, 中科院大类二区top)
6)Li Menghua†; Wu Liyuan†; Chen Mengbing†......Qiao Yuehua*; Ke Zhaoyang*; andShi Xi*; Co-activation of Caspase-1 and Caspase-8 in CMV-induced SGN death by inflammasome-associated pyroptosis and apoptosis, International immunopharmacology, 2022, 113:109305. (SCI, IF=5.714, 中科院大类二区)
7)Wu Liyuan†; Chen Mengbing†; Li Menghua†...... Liu Ke*; Qiao Yuehua*;Shi Xi*. Oridonin alleviates kanamycin-related hearing loss by inhibiting NLRP3/ caspase-1/ gasdermin D-induced inflammasome activation and hair cell pyroptosis. Molecular Immunology, 2022, 149: 66-76. (SCI, IF=4.174, 中科院大类三区 )
8)Li Menghua†; Guo M†; Xu Y† ......Qiao Yuehua*; Ke Zhaoyang*;Shi Xi*. Murine cytomegalovirus employs the mixed lineage kinases family to regulate the spiral ganglion neuron cell death and hearing loss. Neuroscience Letters. 2022 Nov 28:136990. doi: 10.1016/j.neulet.2022.136990. (SCI, IF=3.197, 中科院大类四区)
9)Li Menghua†; Zhang Yan†; Qiu Shiwei†...... Guo Weiwei*; Qiao Yuehua*;Shi Xi*. Oridonin ameliorates noise-induced hearing loss by blocking NLRP3 - NEK7 mediated inflammasome activation. International immunopharmacology, 2021, 95:107576. (SCI, IF=4.932, 中科院大类二区 )
10)Shi Xi†......Wang Xiaohong*. MicroRNA-379 Suppresses Cervical Cancer Cell Proliferation and Invasion by Directly Targeting V-crk Avian Sarcoma Virus CT10 Oncogene Homolog-Like (CRKL)[J]. Oncology Research: Featuring Preclinical and Clinical Cancer Therapeutics, 2018, 26(7): 987-996. (SCI, IF=4.938, 中科院大类二区)
11)Shi Xi†......Qiao Yuehua*. Follicle-stimulating hormone inhibits cervical cancer via nF-κB pathway[J]. OncoTargets and therapy, 2018, 11: 8107. (SCI, IF=4.345, 中科院大类三区)
12)Zhuang Wei†; Wang Caiji†;Shi Xi†.......Qiao Yuehua. MCMV triggers ROS/ NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing loss[J]. International journal of molecular medicine, 2018, 41(6): 3448-3456. (SCI, IF=5.314, 中科院大类三区)
13)Shi Xi†,......Qiao Yuehua*; Liu Ke*. A Novel GJB2 compound heterozygous mutation c. 257C> G (p. T86R)/c. 176del16 (p. G59A fs* 18) causes sensorineural hearing loss in a Chinese family[J]. Journal of clinical laboratory analysis, 2018, 32(7): e22444. (SCI, IF=1.728, 中科院大类四区)
14)Yuan Na†; Qiu Shiwei†......Yang Shiming*; Qiao Yuehua*; andShi Xi*. Hearing analysis in heterozygous and homozygous klotho gene deficient mice[J]. Journal of otology, 2018, 13(4): 131-134. (ESCI )
15)Shi Xi†; Tian Baoqong†......Qiao Yuehua*. GSK3β activity is essential for senescence-associated heterochromatin foci (SAHF) formation induced by HMGA2 in WI38 cells[J]. American journal of translational research, 2017, 9(1): 167. (SCI, IF=3.94, 中科院大类三区)
16)Shi Xi†; Qiu Shiwei†; Zhuang Wei†......Qiao Yuehua*. NLRP3-inflammasomes are triggered by age-related hearing loss in the inner ear of mice[J]. American journal of translational research, 2017, 9(12): 5611. (SCI, IF=3.061, 中科院大类三区)
17)吴俪媛, 郭瑞, 柳柯, 冀飞, 乔月华, &时晰. (2022). 氨基糖苷类抗生素在小鼠耳蜗毛细胞中的分布特征研究. 中华耳科学杂志(020-003).
18)吴俪媛, 陈梦冰, 李梦华, 邱士伟, 乔月华, 张岩,&时晰. (2022). Ednrb基因在waardenburg综合征发病中的作用研究进展. 中华耳科学杂志(020-002).
7.荣誉奖励
江苏省六大人才高峰荣誉称号(2014-2018)
8.其他
授权专利:获已授权发明专利3 项、实用新型专利 1 项、外观设计专利 0 项.
1)柳柯;时晰;Otof 1273 (C>T)基因定点突变的耳聋小鼠模型的构建方法及其应用,2021-11-12,中国,ZL202110894148.4.
2)万美蓉;时晰;一种皮肤支架的延展结构,2022-03-25,中国,ZL202120358738.0.
3)林坚;时晰;陈龙;一种可穿过血迷路屏障的偶联物及其制备方法,2022-06-03,中国,ZL202110566647.0.
4)万美蓉;时晰;去免疫原人脐带内膜-纤维蛋白复合皮肤支架的制备方法,2022-11-11,中国,CN 112891632 B