王大勇男 教授 博士 博士生导师 海南省LJ人才 教育部课程思政教学名师 海南省生物化学与分子生物学会副理事长

邮箱：wangdy@hainanu.edu.cn

通讯地址：海南省海口市美兰区人民大道58号海南大学热带农林主楼817室

邮政编码：570228

1.研究领域

生物技术与分子药理学

2.招生专业

博士：生物化学与分子生物学

硕士：生物化学与分子生物学

3.教育背景

2000-09--2003-07 沈阳药科大学 药理学专业 博士

1997-09--2000-07 黑龙江中医药大学 药理学专业 硕士

1990-09--1995-07 沈阳药科大学 英语药学专业（五年制）学士

4.工作简历

2013-04--今 海南大学 生物化学与分子生物学教授

2012-05--2013-04美国伊利诺依大学农业、消费者及环境科学学院动物科学系 Research Specialist

2010-03--2012-05美国伊利诺依大学分子及细胞生物学院 Research Scientist

2007-07--2010-02 美国伊利诺依大学分子及细胞生物学院 Postdoctoral Research Associate

2007-04--2007-06日本名古屋大学医学部 COE研究员

2004-10--2007-03 日本（原）公定书协会驻名古屋大学附属医院药剂部 Research Resident

2002-04--2004-09 日本名古屋大学大学院神经精神药理学系 外国人研究员

1995-08-2000-08 （原）哈尔滨市医药工业研究所（现）哈药集团生物工程有限公司 新药研发项目负责人

5.讲授课程

研究生高级生物化学、生物化学、生物技术制药、神经生物学、细胞生物学、蛋白质工程

6.目前承担的课题

1）SIRT6-Nrf2蛋白质相互作用与调控及其对基因表达的影响研究（国家自然科学基金），课题负责人

2）调控Keap1与Nrf2相互作用的Keap1蛋白未知磷酸化位点及磷酸化信号传导通路研究（国家自然科学基金），课题负责人

3）与β淀粉样蛋白单体相互作用引起JNK-Tau磷酸化的未知蛋白及其介导的细胞信号传导通路研究（国家自然科学基金），课题负责人

4）基于融合串联抗原表位的新型疫苗治疗热带无爪螨过敏症及其作用机理研究（国家自然科学基金），团队负责人

7.发表论文：

1）Ruijia Fu, Pei Liang, Gang Lu, Jinbao Gu,Dayong Wang*. Prevalence of sparganum infection in wild frogs in Hainan province of China involves a risk for sparganosis.AmericanJournalofTranslationalResearch. 2022; 14(3):1826-1837.

2） YanSun, Baochun Wang, Jinli Pei, Ying Luo, Nan Yuan, Zhengpan Xiao, Hao Wu,Chenghui Luo, Jiaxuan Wang, Shuangshuang Wei, Yechun Pei, Shengmiao Fu*,Dayong Wang*.Molecular dynamic and pharmacological studies on protein-engineered hirudin variants of Hirudinaria manillensis and Hirudo medicinalis.British Journal of Pharmacology.2022 Feb 8. doi: 10.1111/bph.15816.

3）Pei Liang, Xiuji Cui, Ruijia Fu, Peng Liang, Gang Lu,Dayong Wang*. Identification of an enolase gene and its physiological role in Spirometra mansoni.Parasitology Research. 2021, May(25). doi: 10.1007/s00436-021-07175-y.

4）Shuangbao Li, Peng Cao, Congcong Wang, Jianchun Guo, Yuwei Zang, Kunlin Wu, Fangfang Ran, Liangwang Liu,Dayong Wang*, Yi Min*. Genome-wide analysis of tubulin Gene family in cassava and expression of family member FtsZ2-1 during various stress.Plants. 2021, 10:668-685.

5）Jinli Pei, Zhengpan Xiao, Ziyi Guo, Yechun Pei, Shuangshuang Wei, Hao Wu,Dayong Wang*. Sustained stimulation of β2AR inhibits insulin signaling in H9C2 cardiomyoblast cells through the PKA-dependent signaling pathway.Diabetes Metab Syndr Obes. 2020, 2020(13):3887-3898.

6）张转丹, 肖正泮, 武新丽, 孙艳, 罗应, 吴昊, 韦双双, 裴业春*,王大勇*.鱼类神经坏死病毒衣壳蛋白MCP和鮰爱德华氏菌外膜蛋白ompN1融合基因的原核表达. 热带生物学报. 2020; 11(2):145-155.

7）Hao Wu, Shuangshuang Wei, Yonglin Huang, Lintao Chen, Yuerong Wang, Xinli Wu, Zhuandan Zhang, Yechun Pei*,Dayong Wang*. Aβ monomer induces phosphorylation of Tau at Ser-214 through β2AR-PKA-JNK signaling pathway.FASEB Journal. 2020 Apr; 34(4):5092-5105.

8）武新丽, 韦双双, 裴业春, 赵景锋,王大勇*. 反相高效液相色谱法对小鼠血清中基因重组人促肾上腺皮质激素前体及其代谢物的测定. 热带生物学报. 2019, 10(1):83-88.

9）Jinli Pei, Shuangshuang Wei, Yechun Pei, Hao Wu,Dayong Wang*. Role of dietary gluten in development of celiac disease and type I diabetes: management beyond gluten-free diet.Current Medicinal Chemistry. 2020,27(21):3555-3576.

10）Shuangshuang Wei, Yechun Pei, Yuerong Wang, Huai Guan, Yonglin Huang, Tian Xing, Rodney W. Johnson,Dayong Wang*. Role of human Keap1 S53 and S293 residues in modulating the binding of Keap1 to Nrf2.Biochemie. 2019,158:73-81.

11）Yuan Zhou^#, Lintao Chen^#, Xi Zhou, Yechun Pei, Shuangshuang Wei, Anum Mehmood, Yang K. Xiang,Dayong Wang*. Profiling of Differential Expression of Genes in Mice Carrying Both Mutant Presenilin 1 and Amyloid Precursor Protein Transgenes with or without Knockout of Β2 Adrenergic Receptor Gene.Journal of Applied Bioinformatics and Computational Biology. 2018, 7(3):155-168.

12）陈林涛, 韦双双, 黄永林, 吴昊, 郭子怡, 陈银华*,王大勇*. 重组人血清白蛋白在紫花苜蓿中的转基因表达. 热带作物学报. 2018, 39(4):720-725.

13）肖正泮, 刘金莹, 韦双双,王大勇, 裴业春. 重组热带螨Blo t 21变应原诱导小鼠变态反应气道炎症模型的建立, 热带生物学报, 2017, 8 (3):371-376.

14）Yuerong Wang, Shuangshuang Wei, Lintao Chen, Jinli Pei, Hao Wu, Yechun Pei, Yibo Chen,Dayong Wang*. Transcriptomic analysis of gene expression in mice treated with troxerutin.PLOS ONE. 2017, 12(11): e0188261.

15）Yonglin Huang¹, Yechun Pei¹, Huai Guan, Shuangshuang Wei, Hao Wu, Yuan Zhou, Jinli Pei, Lintao Chen, Yuerong Wang, Yibo Chen, Qian Han, Daming Wang,Dayong Wang*. Stability and Pharmacological Effects of Gene-Recombinant Wild Type and Mutant Human Adrenocorticotropic Hormone.Pharmaceutical Research. 2017, 34(4):793-799.

16）肖正泮, 韦双双, 关怀,王大勇, 裴业春*. 热带无爪螨主要变应原Blo t 21抗原表位的预测.热带医学杂志. 2017, 17 (7):841-845.

17）王晓哲, 肖正泮, 韦双双,王大勇, 裴业春*. 利用DNAStar软件预测热带无爪螨主要致敏原Blo t 5的抗原表位.热带生物学报. 2017, 8(1):90-95.

18）黄永林, 韦双双, 裴业春, 吴昊,王大勇*. 基因重组长效人促肾上腺皮质激素药理学活性. 沈阳药科大学学报. 2016, 33(12):996-1000.

19）裴业春, 安晓荣, 侯健, 陈永福, 闫凤祥, 关宏, 韦双双,王大勇*. 猫重组变应原Fel d 1与乙肝病毒核心抗原融合基因的原核表达. 中国畜牧兽医. 2016, 43(11):2826-2833.

20）Dayong Wang, Qin Fu, Yuan Zhou, Qian Shi, Benedict Igwe, Elena V. Wisely, Salvatore Oddo, Yang K. Xiang. β2 adrenergic receptor, protein kinase A (PKA) and c-Jun N-terminal kinase (JNK) signaling pathways mediate tau pathology in Alzheimer’s disease models.Journal of Biological Chemistry. 2013, 288(15):10298-10307.

21）Ruijie Liu,Dayong Wang, Qian Shi, Qin Fu, Steven Hizon, Yang K. Xiang. Palmitoylation Regulates Intracellular Trafficking of β2 Adrenergic Receptor/Arrestin/Phosphodiesterase 4D Complexes in Cardiomyocytes.Plos ONE. 2012,7(8):e42658

22）Dayong Wang, Eunice Yuen, Yuan Zhou, Yan Zhen, Yang K. Xiang. Amyloid beta peptide-(1-42) induces internalization and degradation of beta2 adrenergic receptors in prefrontal cortical neurons.Journal of Biological Chemistry. 2011, 286(36):31852-63.

23）Dayong Wang, Yang K. Xiang. β-adrenergic receptor, amyloid Abeta, and Alzheimer’s disease.Current Topics in Membranes: Advances in Adrenergic Receptor Biology. 2011, 67: 205-229.

24）Dayong Wang, G. Govindaiah, Ruijie Liu, Vania De Arcangelis, Charles Lee Cox, Yang Xiang. Binding of Amyloid βpeptide to β₂adrenergic receptor induces PKA dependent AMPA receptor hyperactivity.FASEBJournal.2010, 24(9):3511-21.

25）Yukihiro Noda, Akihiro Mouri, Yu Ando, Yukari Waki, Shin-nosuke Yamada, Akira Yoshimi, Kiyofumi Yamada, Norio Ozaki,Dayong Wang, Toshitaka Nabeshima. Galantamine ameliorates the impairment of recognition memory in mice repeatedly treated with methamphetamine: involvement of allosteric potentiation of nicotinic acetylcholine receptors and dopaminergic-ERK1/2 systems.The International Journal of Neuropsychopharmacology. 2010, 13(10):1343-54.

26）Hiroko Tsunekawa, Yukihiro Noda, Masayuki Miyazaki, Fumio Yoneda, Toshitaka Nabeshima,Dayong Wang.Effects of (R)-(-)-1-(benzofuran-2-yl)-2-propylaminopentane hydrochloride [(-)-BPAP] in animal models of mood disorders.Behavioural Brain Research.2008, 189(1): 107-116.

27）Dayong Wang, Yukihiro Noda, Hiroko Tsunekawa, Yuan Zhou, Masayuki Miyazaki, Koji Senzaki, Toshitaka Nabeshima. Role of N-Methyl-D-aspartate receptors in antidepressant-like effects of σ₁receptor agonist 1-(3,4-Dimethoxyphenethyl)-4-(3-phenylpropyl)piperazine dihydrochloride(SA-4503) in olfactory bulbectomized rats.Journal of Pharmacology and Experimental Therapeutics. September, 2007, 322(3): 1305-1314.

28）Dayong Wang, Yukihiro Noda, Yuan Zhou, Atsumi Nitta, Hiroshi Furukawa, Toshitaka Nabeshima. Synergistic effect of combined treatment with risperidone and galantamine on phencyclidine-induced impairment of latent visuospatial learning and memory: role of nAChR activation-dependent increase of dopamine D1 receptor-mediated neurotransmission.Neuropharmacology. September, 2007, 53(3):379-89.

29）Dayong Wang, Yukihiro Noda, Yuan Zhou, Akihiro Mouri, Hiroyuki Mizoguchi, Atsumi Nitta, Weiduo Chen, Toshitaka Nabeshima. The allosteric potentiation of nicotinic acetylcholine receptors by galantamine ameliorates the cognitive dysfunction in beta amyloid_25-35i.c.v.-injected mice: involvement of dopaminergic systems.Neuropsychopharmacology. 2007, 32(6): 1261-1271.

30）Dayong Wang, Yukihiro Noda, Yuan Zhou, Atsumi Nitta, Hiroshi Furukawa, Toshitaka Nabeshima. Synergistic effect of galantamine with risperidone on impairment of social interaction in phencyclidine-treated mice as a schizophrenic animal model.Neuropharmacology. 2007, 52(4):1179-87.

31）Dayong Wang, Yukihiro Noda, Hiroko Tsunekawa, Yuan Zhou, Masayuki Miyazaki, Koji Senzaki, Toshitaka Nabeshima. Behavioural and neurochemical features of olfactory bulbectomizedrats resembling depression with comorbid anxiety.Behavioural Brain Research. 2007, 178(2):262-73.

32）王大勇, 于庆海, 周园, Yukihiro Noda, Toshitaka Nabeshima. 现代免疫佐剂研究. 中国免疫学杂志. 2006, 22(2):189-192.

33）周园,王大勇, 王剑楠, 孙凯, 王玉红, 陈维多. 长效基因工程猪α-1干扰素生产工艺的优化研究. 黑龙江畜牧兽医. 2006, 49(6):83-85.

34）周园, 陈维多,王大勇, 苏云明.　重组人α-2b干扰素发酵及粗提条件的优化研究.黑龙江医药.2006, 19(1):35-38.

35）Li-Bo Zou, Akihiro Mouri, Nobuhisa Iwata, Takaomi C. Saido,Dayong Wang, Min-Wei Wang, Hiroyuki Mizoguchi, Yukihiro Noda and Toshitaka Nabeshima. Inhibition of neprilysin by infusion of thiorphan into the hippocampus causes an accumulation of amyloid {beta} and impairment of learning and memory.Journal of Pharmacology and Experimental Therapeutics. 2006, 317(1):334-40.

36）Dayong Wang, Yukihiro Noda, Toshitaka Nabeshima. Features of olfactory bulbectomized rat model of depression and effects of sigma₁receptor agonist.Japanese Journal of Molecular Psychiatry. 2006, 6(2): 81-84.

37）Akihiro Mouri, Li-Bo Zou, Nobuhisa Iwata, Takaomi C. Saido,Dayong Wang,Min-Wei Wang, Yukihiro Noda and Toshitaka Nabeshima. Inhibition of neprilysin by thiorphan (i.c.v.) causes an accumulation of amyloid beta and impairment of learning and memory.Behavioral Brain Research. 2006,168(1):83-91.

38）Takeshi Enomoto, Yukihiro Noda, Akihiro Mouri, Eun-Joo Shin,Dayong Wang, Rina Murai, Kazuo Hotta, Hiroshi Furukawa, Atsumi Nitta, Hyoung-Chun Kim, and Toshitaka Nabeshima. Long-Lasting Impairment of Associative Learning Is Correlated with a Dysfunction of N-Methyl-D-aspartate- Extracellular Signaling-Regulated Kinase Signaling in Mice after Withdrawal from Repeated Administration of Phencyclidine.Molecular Pharmacology. 2005,68:1765-1774.

39）Hiroko Tsunekawa, Yukihiro Noda,Dayong Wang, Hiroyuki Mouri, Toshitaka Nabeshima. Selegiline potentiates effects of donepezil on cognitive impairment induced by amyloid beta (25-35).Bulletin of the Japanese Society for Neurochemistry.2005, 44: 182.

40）Dayong Wang, Yukihiro Noda, Yuan Zhou, Atsumi Nitta, Toshitaka Nabeshima and Qinghai Yu. Effects of sodium houtuyfonate on phosphorylation of CaMK II, CREB and ERK 1/2 and expression of c-Fos in macrophages.International Immunopharmacology.2004, 8: 1083-1088.

41）Tsunekawa Hiroko, Yukihiro Noda,Dayong Wang, Toshitaka Nabeshima. Antidepressive effect of(R)-(-)-1-(benzofuran-2-yl)-2-propylaminopentane hydrochloride [(-)-BPAP] in animal models of stress disorders.Journal of Pharmacological Science. 2004, 94 (suppl.): 225P.(Abstract)

42）王大勇, 毕秀丽, 周园, 彭缨, 野田幸裕, 锅岛俊隆. 合成鱼腥草素对巨噬细胞呼吸爆发、细胞内游离钙离子浓度及T细胞分泌白细胞介素-2的影响.沈阳药科大学学报. 2003, 20(3):210-214.

43）朱晔, 毕鸿亮, 王忠, 林竹青,王大勇. 复方马来酸依那普利片含量的双波长HPLC法测定.黑龙江医药. 2003, 16(2):9-10.

44）Dayong Wang, Qinghai Yu, Peggy Eikstadt, Darcy Hammond, Yun Feng, and Na Chen. Studies on adjuvaticity of sodium houttuyfonate and its mechanism.International Immunopharmacology.2002, 10:1411-1418.

45）Dayong Wang, Qinghai Yu, Yun Feng, and Na Chen. Adjuvanticity of sodium houttuyfonate.Indian Journal of Pharmacology. 2002, 34(5):363-364.

46）周园,王大勇, 雷敏. HPLC法测定复方依那普利片累计溶出度.《黑龙江医药》2002, 15(2):91-92.

47）王立强, 丁一兵, 周园,王大勇. 6,7-二甲氧基香豆素对豚鼠心房DME及家兔胃电的影响.沈阳药科大学学报. 2001, 18(增刊): 87-88.

48）孟庆林, 王立强, 聂凤文, 周园,王大勇. 茵陈胶囊降血脂作用实验研究.沈阳药科大学学报. 2001, 18(增刊):89-90.

49）.周慧秋, 于滨, 乔万红,王大勇, 张万荣, 苏云明. 甲基丁香酚药理作用研究.中医药学报. 2000, 28(2):79-80.

50）王大勇,于滨, 李国玉. 胃养消胶囊药理作用研究.黑龙江医药. 2000, 13(2): 96-97.

51）苏云明,王大勇, 赵伟群, 张万荣, 白云, 周慧秋, 于滨. 芦荟对大鼠血清胰高血糖素水平的影响.中医药学报. 1999, 27(6): 51.

52）王忠, 毕鸿亮,王大勇. 马来酸依那普利片剂溶出度考察. 黑龙江医药. 1997, 10(1): 11-13.

8.申报的发明专利

1）神经坏死病毒MCP与鮰爱德华氏菌ompN1的融合蛋白及制备方法. CN2019102663878

2）一种基因重组人促皮质素与白蛋白的融合蛋白及表达方法. CN201910186462X

3）一组提高血清糖皮质激素水平的基因重组人促皮质素前体及制备方法.

4）一种用柱花草表达的基因重组人血清白蛋白及表达方法. CN2018107097504

5）一种用黑麦草表达的基因重组人血清白蛋白及表达方法. CN2017113377349

6）一种用紫花苜蓿表达的基因重组人血清白蛋白及表达方法. CN2017113362644

7）促皮质素与胰岛素样生长因子1融合蛋白及其制备方法. CN2017110746869

8）热带无爪螨重组变应原Blo t 5-21、其制备方法及过敏小鼠模型的建立. CN2017105789968

9）一种基因重组长效人促肾上腺皮质激素及其制备方法. CN2016100837725

10）一种展示重组猫致敏原rFel d 1 蛋白的免疫增强型病毒样颗粒、其表达载体及其制备与应用. CN 2015107584450

9.成果与奖励

1）（原）美国National Alliance of Research on Schizoprenia and Depression (NARSAD) 2009青年研究员奖

2）美国伊利诺伊大学分子及细胞生物学院学术论坛一等奖

3）主持完成美国脑与行为研究基金会项目

4）主持完成美国伊利诺依州公众卫生部阿尔茨海默病研究基金项目

5）主持完成国家和省自然科学基金项目