团队名称：热带大豆分子育种与种质创新团队

团队负责人：

李海燕，女，1971年出生，海南大学热带作物学院二级教授，博士生导师，国务院政府特殊津贴专家（2016）。目前担任教育部生物科学类专业教学指导委员会委员，中国植物生理与分子生物学学会女科学家分会理事，中国生物化学与分子生物学学会农学分会理事，吉林省大豆品种审定委员会委员。近年来，主持国家“863”计划课题、国家转基因重大专项子课题、国家重点研发计划任务、国家自然科学基金等课题20余项；在The Plant Journal等杂志发表SCI论文80余篇；授权发明专利23件；以第一或第二完成人获部省级科技进步一等奖等科研奖励7项。

团队研究方向：

本团队长期以来一直致力于大豆种质资源精准鉴定、基因挖掘与分子育种研究工作。主要研究方向：(1)热带大豆/毛豆优异种质资源表型与基因型精准鉴定；(2)热带大豆/毛豆抗逆、高产优异基因挖掘与分子育种。近年来，挖掘到一系列大豆耐高温、耐盐碱、抗旱及控制产量与生长发育等相关基因，创制了四粒荚、大粒、高产等大豆新种质200余份。

团队代表性成果：

发表的主要学术论文：

1.Chen K, Su C, Tang WS, Zhou YB, Xu ZS, Chen J,Li HY*, Chen M*, Ma YZ*.Nuclear transport factor GmNTF2B-1 enhances soybean drought tolerance by interacting with oxidoreductase GmOXR17 to reduce ROS content.The plant Journal,2021,107(3): 740-759.

2.Wenping Zhang^(#), Jishan Lin^(#), Jianguo Li^(#), Shaoquan Zheng^(#),……Ming Ray. Rambutan genome revealed gene networks for spine formation and aril development.The Plant Journal.2021,108, 1037–1052.

3.Xu KH, Zhao Y, Zhao Y, Feng C, Zhang YH, Wang FW, Li XW, Gao HT, Liu WC, Jing Y, Saxena RK, Feng XZ, Zhou YG*,Li HY*.Soybean F-Box-Like Protein GmFBL144 Interacts with Small Heat Shock Protein and Negatively Regulates Plant Drought Stress Tolerance.Frontiers in Plant Science,2022,2(13):823529.

4.Zhou YG, Sun M, Sun P, Gao H, Yang H, Jing Y, Hussain MA, Saxena RK, Carther FI, Wang Q,Li HY*. Tonoplast inositol transporters: Roles in plant abiotic stress response and crosstalk with other signals.Journal of Plant Physiology, 2022, 271:153660.

5.Zhao X^#,Jing Y^#, Luo ZH, et al. GmST1, which encodes a sulfotransferase, confers resistance to soybean mosaic virus strains G2 and G3.Plant Cell and Environment, 2021, 04.

6.Jing Y, Teng WL, Qiu LJ, et al. Genetic dissection of soybean partial resistance to sclerotinia stem rot through genome wide association study and high throughout single nucleotide polymorphisms.Genomics,2021, 113.

7.Gao HT^#, Wang FW^#, Hu XL, Li YL, Zhang Y, Kue Foka IC, Wang B, Min F, Wang X, Wu H, Xu KH, Zhou YG, Liu X*, Li XK*,Li HY*.Camelina lipid droplets as skin delivery system promotes wound repair by enhancing the absorption of hFGF2.International Journal of Pharmaceutics,2021, 598(3):120327.

8.Feng C, He C, Wang Y, Xu H, Xu K, Zhao Y, Yao B, Zhang Y, Zhao Y, Idrice Carther KF, Luo J, Sun D, Gao H, Wang F, Li X, Liu W, Dong Y, Wang N, Zhou Y,Li HY*. Genome-wide identification of soybean Shaker K⁺channel gene family and functional characterization of GmAKT1 in transgenicArabidopsis thalianaunder salt and drought stress.Journal of Plant Physiology. 2021, 266:153529.

9.Ketehouli T^#, Zhou YG^#, Dai SY, Sun DQ, Li Y, Hu X, Wang FW, Liu WC, Li XW*,Li HY*. A soybean calcineurin B-like protein-interacting protein kinase, GmPKS4, regulates plant responses to salt and alkali stresses.Journal of Plant Physiology,2021, 153331.

10.Xu KH, Wu N, Yao WB, Li XW, Zhou YG,Li HY*. The Biological Function and Roles in Phytohormone Signaling of the F-Box Protein in Plants.Agronomy. 2021, 11: 2360.

11.Chen K, Tang WS, Zhou YB, Xu ZS, Chen J, Ma YZ, Chen M* andLi HY*. Overexpression ofGmUBC9gene enhances plant drought resistance and affects flowering time via histone H2B monoubiquitination.Frontiers in Plant Science, 2020, 11:555794.

12.Naveed A, Li T, Liu Y, Nguyen QVH, Ma X, Zhang X, Liu JY, Yao N, Liu XM^*,Li HY^*. Molecular and biochemical rhythms in dihydroflavonol 4-reductase -mediated regulation of leucoanthocyanidin biosynthesis inCarthamus tinctoriusL.Industrial Crops & Products, 2020, 156:112838.

13.Zhou YG, Liu WC, Li XW, Sun DQ, Xu KH, Feng C, Kue Foka IC, Ketehouli T, Gao HT, Wang N, Dong YY, Wang FW*,Li HY*.Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance viaGmCSDsandGmCCSin transgenic Arabidopsis and soybean.BMC Plant Biology,2020, 20:190.

14.Carther KFI, Ketehouli T, Ye N, Yang YH, Wang N, Dong YY, Yao N, Liu XM, Liu WC, Li XW, Wang FW*,Li HY*.Comprehensivegenomicanalysis andexpressionprofiling ofDiacylglycerolKinase (DGK)genefamily in Soybean (Glycine max) under Abiotic Stresses.InternationalJournalof molecular science, 2019, 20:1361.

15.Muhammad N, Jameel A, Qiang WD, Ahmad N, Liu WC, Wang FW*,Li HY*.Overexpression ofGmCAMTA12enhanceddroughttolerance in Arabidopsis and Soybean. InternationalJournalof molecular science, 2019, 20, 1361.

16.Jameel A, Muhammad N, Wang FW, Liu WC, Zhou YG, Li HY*, Li XW*.Synthetic promoters: designing the cis regulatory modules for controlled gene expression. Molecular biotechnology, 2018, 60(8):608-620. (通讯作者)

17.Jing Y^#, Zhao X^#, WangJY^#, et al. Identification of the Genomic Region Underlying Seed Weight per Plant in Soybean (Glycine max L. Merr.) via High-Throughput Single-Nucleotide Polymorphisms and a Genome-Wide Association Study.Frontiers in Plant Science,2018, 9.

18.Li XW, WangFW, Sun DQ, Dong YY,Li HY*.Cloning and characterization of SucNHX1, a novel vacuolar Na+/H+ antiporter from the halophyte S. corniculata that enhances the saline-alkali tolerance in Arabidopsis by its overexpression.Plant Cell, Tissue and Organ Culture, 2018.

19.Liu WC, Zhou YG, Li XW, Wang XC, Dong YY, Wang N, Liu XM, Chen H, Yao N, Cui XY, Jameel A, Wang FW*,Li HY*. Tissue-specific regulation of Gma-miR396 family on coordinating development and low water availability responses.Frontiers in Plant Science,2017,8: 1112.

20.Zong JM, Li XW, Zhou YH, Wang FW, Wang N, Dong YY, Yuan YX, Chen H, Liu XM, Yao N,Li HY*. The AaDREB1 transcription factor from the cold-tolerant plant Adonis amurensis enhances Abiotic stress tolerance in Transgenic Plant.InternationalJournalof molecular science,2016, 17(4):611.

21.Liu WC, Deng Y, Zhou YG, Chen H, Dong YY, Wang N, Li XW, Jameel A, Yang H, Zhang M, Chen K, Wang FW*,Li HY*. Normalization for relative quantification of mRNA and microRNA in soybean exposed to various abiotic stresses.PLoS One,2016,11(5): e0155606.

22.Wang FW, Chen H, Li XW, Wang N, Wang TY, Yang J, Yao N, Du LN, Wang YF, Liu XM, Chen X, Wang ZM, Dong YY*,Li HY*. Mining and identification of polyunsaturated fatty acid synthesis genes active during camelina seed development using 454 pyrosequencing.BMC Plant Biology,2015,15: 147.

23.Wang FW, Deng Y, Zhou YG, Dong JY, Chen H, Dong YY, Wang N, Li XW,Li HY*. Genome-Wideanalysis andexpressionprofiling of thephospholipase Cgenegamily in Soybean (Glycine max).PLoS One,2015, 10(9): e0138467.

24.Chen H, Wang FW, Dong YY, Wang N, Sun YP, Li XY, Liu L, Fan XD, Yin HL, Jing YY, Zhang XY, Li YL, Chen G,Li HY*.Sequence mining and transcript profiling to explore differentially expressed genes associated with lipid biosynthesis during soybean seed development.BMC Plant Biology,2012, 31(12):122.

25.Liu L, Wang Y, Wang N, Dong YY, Fan XD, Liu XM, Yang J,Li HY*.Cloning of a vacuolar H (+)-pyrophosphatase gene from the halophyte Suaeda corniculata whose heterologous overexpression improves salt, saline-alkali and drought tolerance in Arabidopsis.Journal of Integrative Plant Biology,2011, 53(9):731-742.

授权的主要发明专利：

（1）李海燕，李晓薇，王法微，刘伟灿，王南，董园园，刘秀明，姚娜，刘欣，肖洪庆，侯心悦.大豆低温诱导人工合成启动子SP5及其应用，2020-07-24，中国，ZL201710312593.9.c

（2）李海燕，李晓薇，王法微，刘伟灿，王南，董园园，刘秀明，姚娜，刘欣，肖洪庆，戢舒涵.一种植物干旱诱导型人工合成启动子SP2及应用，2020-07-24，中国，ZL201710312594.3.

（3）李海燕，王法微，张 洁，王南，李琼琼，董圆圆，陈欢.大豆逆境诱导基因启动子及其应用，2015-09-30，ZL201410045503.0.

（4）李海燕，王佳琦，张晓美，王南，陈欢.一种高频诱导大豆丛生芽的高效转基因方法，2012-10-31，中国，ZL201010584615.5.

（5）李海燕，崔喜艳，刘晓庆，张林波，陈展宇，宋慧.一种大豆叶片特异性启动子SRS4及其应用，2012-01-11，中国，ZL201010526255.3.

（6）李海燕，刘 亮，王 南，王莹.一种植物耐高pH盐碱基因SucHP及应用，2012-10-31，中国，ZL200810050629.1.

获得的主要科研奖励：

（1）生长因子类蛋白植物油体生物反应器研制，吉林省技术发明一等奖，排名：第一，时间：2019.12.

（2）植物重要基因资源挖掘与大豆特异种质创制及新品种选育，吉林省科技进步一等奖，排名：第一，时间：2014.12.

（3）吉林省青年科技奖，时间：2014.8.

（4）植物抗逆基因挖掘及其调控机制研究，吉林省自然科学学术成果一等奖，排名：第一，时间：2012.10.

（5）大豆重要功能基因的克隆、高产优质新品种的选育及区域丰产增效栽培技术研究，中国作物科技奖，排名：第二，时间：2016.07.